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rat bone marrow derived mesenchymal stem cells bmscs  (Servicebio Inc)

 
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    Servicebio Inc rat bone marrow derived mesenchymal stem cells bmscs
    PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. (C) TGF‐β1 (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat <t>bone</t> <t>marrow‐derived</t> <t>BMSCs</t> were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.
    Rat Bone Marrow Derived Mesenchymal Stem Cells Bmscs, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+bone+marrow+derived+mesenchymal+stem+cells+bmscs/cells+endothelial+human+huvecs+umbilical+vein/pmc12862434-361-0-17
    Average 86 stars, based on 1 article reviews
    rat bone marrow derived mesenchymal stem cells bmscs - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Periodic Therapeutic Phlebotomy Mitigates Systemic Aging Phenotypes by Promoting Bone Marrow Function"

    Article Title: Periodic Therapeutic Phlebotomy Mitigates Systemic Aging Phenotypes by Promoting Bone Marrow Function

    Journal: Aging Cell

    doi: 10.1111/acel.70400

    PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. (C) TGF‐β1 (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. (C) TGF‐β1 (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.

    Techniques Used: Transformation Assay, Staining, Fluorescence, Flow Cytometry, Derivative Assay, Cell Culture, MTT Assay, Western Blot, Expressing, Cell Cycle Assay

    Related Articles

    Isolation:

    Article Title: Periodic Therapeutic Phlebotomy Mitigates Systemic Aging Phenotypes by Promoting Bone Marrow Function
    Article Snippet: The primary antibodies used for Western blotting included: p53 (Wanleibio, WL01919), p27 (Wanleibio, WL04174), p21 (Wanleibio, WL0362), p16 (Wanleibio, WL01418), p‐tau (Abclonal, AP1096), tau‐5 (Affinity, AF6141), NEFL (Proteintech, 12998‐1‐AP), SIRT2 (Proteintech, 19655‐1‐AP), GH1 (Proteintech, 55243‐1‐AP), IGF (Abclonal, A11985), and β‐actin (Wanleibio, WL01372). .. Rat bone marrow‐derived mesenchymal stem cells (BMSCs) were isolated and cultured in Dulbecco's Modified Eagle's Medium (DMEM, Servicebio, G4510) supplemented with 1% fetal bovine serum (FBS, TIANHANG, 11011‐8611) at 37°C in a 5% CO 2 atmosphere. .. The primary antibodies used for Western blotting included: p53 (Wanleibio, WL01919), p27 (Wanleibio, WL04174), p21 (Wanleibio, WL0362), p16 (Wanleibio, WL01418), p‐tau (Abclonal, AP1096), tau‐5 (Affinity, AF6141), NEFL (Proteintech, 12998‐1‐AP), SIRT2 (Proteintech, 19655‐1‐AP), GH1 (Proteintech, 55243‐1‐AP), IGF (Abclonal, A11985), and β‐actin (Wanleibio, WL01372).

    Cell Culture:

    Article Title: Periodic Therapeutic Phlebotomy Mitigates Systemic Aging Phenotypes by Promoting Bone Marrow Function
    Article Snippet: The primary antibodies used for Western blotting included: p53 (Wanleibio, WL01919), p27 (Wanleibio, WL04174), p21 (Wanleibio, WL0362), p16 (Wanleibio, WL01418), p‐tau (Abclonal, AP1096), tau‐5 (Affinity, AF6141), NEFL (Proteintech, 12998‐1‐AP), SIRT2 (Proteintech, 19655‐1‐AP), GH1 (Proteintech, 55243‐1‐AP), IGF (Abclonal, A11985), and β‐actin (Wanleibio, WL01372). .. Rat bone marrow‐derived mesenchymal stem cells (BMSCs) were isolated and cultured in Dulbecco's Modified Eagle's Medium (DMEM, Servicebio, G4510) supplemented with 1% fetal bovine serum (FBS, TIANHANG, 11011‐8611) at 37°C in a 5% CO 2 atmosphere. .. The primary antibodies used for Western blotting included: p53 (Wanleibio, WL01919), p27 (Wanleibio, WL04174), p21 (Wanleibio, WL0362), p16 (Wanleibio, WL01418), p‐tau (Abclonal, AP1096), tau‐5 (Affinity, AF6141), NEFL (Proteintech, 12998‐1‐AP), SIRT2 (Proteintech, 19655‐1‐AP), GH1 (Proteintech, 55243‐1‐AP), IGF (Abclonal, A11985), and β‐actin (Wanleibio, WL01372).

    Modification:

    Article Title: Periodic Therapeutic Phlebotomy Mitigates Systemic Aging Phenotypes by Promoting Bone Marrow Function
    Article Snippet: The primary antibodies used for Western blotting included: p53 (Wanleibio, WL01919), p27 (Wanleibio, WL04174), p21 (Wanleibio, WL0362), p16 (Wanleibio, WL01418), p‐tau (Abclonal, AP1096), tau‐5 (Affinity, AF6141), NEFL (Proteintech, 12998‐1‐AP), SIRT2 (Proteintech, 19655‐1‐AP), GH1 (Proteintech, 55243‐1‐AP), IGF (Abclonal, A11985), and β‐actin (Wanleibio, WL01372). .. Rat bone marrow‐derived mesenchymal stem cells (BMSCs) were isolated and cultured in Dulbecco's Modified Eagle's Medium (DMEM, Servicebio, G4510) supplemented with 1% fetal bovine serum (FBS, TIANHANG, 11011‐8611) at 37°C in a 5% CO 2 atmosphere. .. The primary antibodies used for Western blotting included: p53 (Wanleibio, WL01919), p27 (Wanleibio, WL04174), p21 (Wanleibio, WL0362), p16 (Wanleibio, WL01418), p‐tau (Abclonal, AP1096), tau‐5 (Affinity, AF6141), NEFL (Proteintech, 12998‐1‐AP), SIRT2 (Proteintech, 19655‐1‐AP), GH1 (Proteintech, 55243‐1‐AP), IGF (Abclonal, A11985), and β‐actin (Wanleibio, WL01372).



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    90
    Dawley Inc bone marrow mesenchymal stem cells (bmscs) derived from male sprague–dawley (sd) rats
    PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. (C) TGF‐β1 (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat <t>bone</t> <t>marrow‐derived</t> <t>BMSCs</t> were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.
    Bone Marrow Mesenchymal Stem Cells (Bmscs) Derived From Male Sprague–Dawley (Sd) Rats, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+bone+marrow+derived+mesenchymal+stem+cells+bmscs/bone+mesenchymal+stem+cells++mscs+/10__1039_slash_c8tb01001c-61-11-9
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    bone marrow-derived mesenchymal stem cells ( bmscs ) isolated from 3–4 weeks of sprague-dawley (sd) rats  (Dawley Inc)
    90
    Dawley Inc bone marrow-derived mesenchymal stem cells ( bmscs ) isolated from 3–4 weeks of sprague-dawley (sd) rats
    PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. (C) TGF‐β1 (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat <t>bone</t> <t>marrow‐derived</t> <t>BMSCs</t> were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.
    Bone Marrow Derived Mesenchymal Stem Cells ( Bmscs ) Isolated From 3–4 Weeks Of Sprague Dawley (Sd) Rats, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+bone+marrow+derived+mesenchymal+stem+cells+bmscs/bone+marrow+mesenchymal+stem+cells++bmscs+/pmc05428835-203-15-13
    Average 90 stars, based on 1 article reviews
    bone marrow-derived mesenchymal stem cells ( bmscs ) isolated from 3–4 weeks of sprague-dawley (sd) rats - by Bioz Stars, 2026-10
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    PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. (C) TGF‐β1 (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Aging Cell

    Article Title: Periodic Therapeutic Phlebotomy Mitigates Systemic Aging Phenotypes by Promoting Bone Marrow Function

    doi: 10.1111/acel.70400

    Figure Lengend Snippet: PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. (C) TGF‐β1 (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Rat bone marrow‐derived mesenchymal stem cells (BMSCs) were isolated and cultured in Dulbecco's Modified Eagle's Medium (DMEM, Servicebio, G4510) supplemented with 1% fetal bovine serum (FBS, TIANHANG, 11011‐8611) at 37°C in a 5% CO 2 atmosphere.

    Techniques: Transformation Assay, Staining, Fluorescence, Flow Cytometry, Derivative Assay, Cell Culture, MTT Assay, Western Blot, Expressing, Cell Cycle Assay